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31.
32.
Malika Faouzi Frederic Hague Marie Potier Ahmed Ahidouch Henri Sevestre Halima Ouadid‐Ahidouch 《Journal of cellular physiology》2011,226(2):542-551
Breast cancer (BC) is the leading cancer in the world in terms of incidence and mortality in women. However, the mechanism by which BC develops remains largely unknown. The increase in cytosolic free Ca2+ can result in different physiological changes including cell growth and death. Orai isoforms are highly Ca2+ selective channels. In the present study, we analyzed Orai3 expression in normal and cancerous breast tissue samples, and its role in MCF‐7 BC and normal MCF‐10A mammary epithelial cell lines. We found that the expression of Orai3 mRNAs was higher in BC tissues and MCF‐7 cells than in normal tissues and MCF‐10A cells. Down‐regulation of Orai3 by siRNA inhibited MCF‐7 cell proliferation and arrested cell cycle at G1 phase. This phenomenon is associated with a reduction in CDKs 4/2 (cyclin‐dependent kinases) and cyclins E and D1 expression and an accumulation of p21Waf1/Cip1 (a cyclin‐dependent kinase inhibitor) and p53 (a tumor‐suppressing protein). Orai3 was also involved in MCF‐7 cell survival. Furthermore, Orai3 mediated Ca2+ entry and contributed to intracellular calcium concentration ([Ca2+]i). In MCF‐10A cells, silencing Orai3 failed to modify [Ca2+]i, cell proliferation, cell‐cycle progression, cyclins (D1, E), CDKs (4, 2), and p21Waf1/Cip1 expression. Our results provide strong evidence for a significant effect of Orai3 on BC cell growth in vitro and show that this effect is associated with the induction of cell cycle and apoptosis resistance. Our study highlights a possible role of Orai3 as therapeutic target in BC therapy. J. Cell. Physiol. 226: 542–551, 2011. © 2010 Wiley‐Liss, Inc. 相似文献
33.
Catherine Thèves Alice Senescau Stefano Vanin Christine Keyser Fran?ois Xavier Ricaut Anatoly N. Alekseev Henri Dabernat Bertrand Ludes Richard Fabre Eric Crubézy 《PloS one》2011,6(7)
Research of ancient pathogens in ancient human skeletons has been mainly carried out on the basis of one essential historical or archaeological observation, permitting specific pathogens to be targeted. Detection of ancient human pathogens without such evidence is more difficult, since the quantity and quality of ancient DNA, as well as the environmental bacteria potentially present in the sample, limit the analyses possible. Using human lung tissue and/or teeth samples from burials in eastern Siberia, dating from the end of 17th to the 19th century, we propose a methodology that includes the: 1) amplification of all 16S rDNA gene sequences present in each sample; 2) identification of all bacterial DNA sequences with a degree of identity ≥95%, according to quality criteria; 3) identification and confirmation of bacterial pathogens by the amplification of the rpoB gene; and 4) establishment of authenticity criteria for ancient DNA. This study demonstrates that from teeth samples originating from ancient human subjects, we can realise: 1) the correct identification of bacterial molecular sequence signatures by quality criteria; 2) the separation of environmental and pathogenic bacterial 16S rDNA sequences; 3) the distribution of bacterial species for each subject and for each burial; and 4) the characterisation of bacteria specific to the permafrost. Moreover, we identified three pathogens in different teeth samples by 16S rDNA sequence amplification: Bordetella sp., Streptococcus pneumoniae and Shigella dysenteriae. We tested for the presence of these pathogens by amplifying the rpoB gene. For the first time, we confirmed sequences from Bordetella pertussis in the lungs of an ancient male Siberian subject, whose grave dated from the end of the 17th century to the early 18th century. 相似文献
34.
Christian Mulder J. Arie Vonk Henri A. Den Hollander A. Jan Hendriks Anton M. Breure 《Oikos》2011,120(4):529-536
For most species, the logarithm of their average body mass is negatively related to the logarithm of their relative population density, i.e. the numerical abundance. In this way, the allometric scaling (both mass–abundance regressions and body–size spectra) becomes useful in ecological theory to build and explain food webs. Using empirical evidence derived from 145 Dutch sites, a hypothesis is formulated to explain how soil microbivores, detritivores and predators react to increasing resource availability. Shifts in size distribution, and subsequently changes in soil food‐web structure, are further discussed in the perspective of Holling's sequential interactions between basic system functions. We show that the allometric scaling and the averages of the (log‐transformed) prey:predator body‐mass ratios are reliable predictors for assessing faunal responses to nutrient availability. We view this work as a first attempt toward an extensive comparison of ecological processes in different soil systems. 相似文献
35.
36.
Henri‐Obadja Kumada Jeffrey‐Tri Nguyen Taeko Kakizawa Koushi Hidaka Tooru Kimura Yoshio Hayashi Yoshiaki Kiso 《Journal of peptide science》2011,17(8):569-575
HTLV‐I is a debilitating and/or lethal retrovirus that causes HTLV‐I‐associated myelopathy/tropical spastic paraparesis, adult T‐cell leukemia and several inflammatory diseases. HTLV‐I protease is an aspartic retropepsin involved in HTLV‐I replication and its inhibition could treatHTLV‐I infection. A recombinant L40I mutant HTLV‐I protease was designed and obtained from Escherichia coli, self‐processingand purification by ion‐exchange chromatography. The protease was refolded by a one‐step dialysis and recovered activity. The cleavage efficiency of the [Ile40]HTLV‐I protease was at least 300 times higher for a fluorescent substratethan that of our previously reported recombinant His‐tagged non‐mutated HTLV‐I protease. In addition, we designed and synthesized a substrate containing a highly fluorescent Mca moiety in the fragment before the scissile bond, and a chromogenic p‐nitrophenylalanine moiety after the scissile bond that greatly amplified spectrometry detection and improved the HTLV‐I protease inhibition potency assay. The HTLV‐I protease inhibition assay with the [Ile40]HTLV‐I protease and fluorogenic substrate requires distinctively less protease, substrate, inhibitor and assay time than our previous methods. This means our new assay is more cost‐effective and more time‐efficient while being reproducible and less labor‐intensive. Copyright © 2011 European Peptide Society and John Wiley & Sons, Ltd. 相似文献
37.
38.
Sylvain de
Breyne Caroline Vindry Olivia Guillin Lionel Cond Fabrice Mure Henri Gruffat Laurent Chavatte Thophile Ohlmann 《Nucleic acids research》2020,48(22):12502
Coronaviruses represent a large family of enveloped RNA viruses that infect a large spectrum of animals. In humans, the severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2) is responsible for the current COVID-19 pandemic and is genetically related to SARS-CoV and Middle East respiratory syndrome-related coronavirus (MERS-CoV), which caused outbreaks in 2002 and 2012, respectively. All viruses described to date entirely rely on the protein synthesis machinery of the host cells to produce proteins required for their replication and spread. As such, virus often need to control the cellular translational apparatus to avoid the first line of the cellular defense intended to limit the viral propagation. Thus, coronaviruses have developed remarkable strategies to hijack the host translational machinery in order to favor viral protein production. In this review, we will describe some of these strategies and will highlight the role of viral proteins and RNAs in this process. 相似文献
39.
Henri Weimerskirch Matthieu Le Corre Hélène Gadenne David Pinaud Akiko Kato Yan Ropert-Coudert Charles-André Bost 《Oecologia》2009,161(3):637-649
Reversed sexual dimorphism (RSD) may be related to different roles in breeding investment and/or foraging, but little information
is available on foraging ecology. We studied the foraging behaviour and parental investment by male and female masked boobies,
a species with RSD, by combining studies of foraging ecology using miniaturised activity and GPS data loggers of nest attendance,
with an experimental study where flight costs were increased. Males attended the chick more often than females, but females
provided more food to the chick than males. Males and females foraged during similar periods of the day, had similar prey
types and sizes, diving depths, durations of foraging trips, foraging zones and ranges. Females spent a smaller proportion
of the foraging trip sitting on the water and had higher diving rate than males, suggesting higher foraging effort by females.
In females, trip duration correlated with mass at departure, suggesting a flexible investment through control by body mass.
The experimental study showed that handicapped females and female partners of handicapped males lost mass compared to control
birds, whereas there was no difference for males. These results indicate that the larger female is the main provisioner of
the chick in the pair, and regulates breeding effort in relation to its own body mass, whereas males have a fixed investment.
The different breeding investment between the sexes is associated with contrasting foraging strategies, but no clear niche
differentiation was observed. The larger size of the females may be advantageous for provisioning the chick with large quantities
of energy and for flexible breeding effort, while the smaller male invests in territory defence and nest guarding, a crucial
task when breeding at high densities. In masked boobies, division of labour appears to be maximal during chick rearing—the
most energy-demanding period—and may be related to evolution of RSD. 相似文献
40.
Pollen dispersal in sugar beet production fields 总被引:1,自引:0,他引:1
Henri Darmency Etienne K. Klein Thierry Gestat De Garanbé Pierre-Henri Gouyon Marc Richard-Molard Claude Muchembled 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2009,118(6):1083-1092
Pollen-mediated gene flow has important implications for biodiversity conservation and for breeders and farmers’ activities.
In sugar beet production fields, a few sugar beet bolters can produce pollen as well as be fertilized by wild and weed beet.
Since the crop, the wild beets, and the weed beets are the same species and intercross freely, the question of pollen flow
is an important issue to determine the potential dispersal of transgenes from field to field and to wild habitats. We report
here an experiment to describe pollen dispersal from a small herbicide-resistant sugar beet source towards male sterile target
plants located along radiating lines up to 1,200 m away. Individual dispersal functions were inferred from statistical analyses
and compared. Pollen limitation, as expected in root-production fields, was confirmed at all the distances from the pollen
source. The number of resistant seeds produced by bait plants best fitted a fat-tailed probability distribution curve of pollen
grains (power–law) dependent on the distance from the pollen source. A literature survey confirmed that power–law function
could fit in most cases. The b coefficient was lower than 2. The number of fertilized flowers by background (herbicide-susceptible) pollen grains was uniform
across the whole field. Airborne pollen had a fertilization impact equivalent to that of one adjacent bolter. The individual
dispersal function from different pollen sources can be integrated to provide the pollen cloud composition for a given target
plant, thus allowing modeling of gene flow in a field, inter-fields in a small region, and also in seed-production area. Long-distance
pollen flow is not negligible and could play an important role in rapid transgene dispersal from crop to wild and weed beets
in the landscape. The removing of any bolting, herbicide-resistant sugar beet should be compulsory to prevent the occurrence
of herbicide-resistant weed beet, thus preventing gene flow to wild populations and preserving the sustainable utility of
the resistant varieties. Whether such a goal is attainable remains an open question and certainly would be worth a large scale
experimental study. 相似文献